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spcas9 coding region  (Addgene inc)


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    Addgene inc spcas9 coding region
    (A) Table of the Cas9 orthologs utilized in this work, showing their size (amino acid) and PAM sequence (5’). (B) Schematic size comparisons of the panel of Cas9 orthologs tested with <t>SpCas9</t> and other compact CRISPR systems commonly used for in vivo genome editing. (C) Schematic outlining the experimental steps to evaluate Cas9 ortholog editing efficiency by tdTomato knock-out (KO), quantified by flow cytometry analysis. PiggyBac constructs encode for: ortholog-specific hU6-sgRNA cassettes and puromycin selection gene; doxycycline-inducible Cas9 orthologs and hygromycin selection gene. (D–E) Comparison of SpCas9 cleavage efficiency with Cco, Cme2, Gsp, Msc, Nsp, Sdo and Tmo Cas9 orthologs using three ortholog-specific spacer sequences targeting the tdTomato gene in mESC (D) and HEK293T cells (E). Editing efficiencies at individual target sites are shown in the left panels, while mean editing efficiencies for each Cas9 ortholog across the three sites are presented in the right panels. (F) Heatmaps showing editing efficiencies of Cme2 (left) and Msc (right) using twelve distinct sgRNAs, quantified by flow cytometry. Asterisks indicate P-values from unpaired t -test comparing three independent biological replicates to the respective controls. *P < 0.05. Error bars ± SD.
    Spcas9 Coding Region, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 368 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/spcas9+coding+region/bio_rxiv__64898__2026__01__12__698990-197-0-10?v=Addgene+inc
    Average 96 stars, based on 368 article reviews
    spcas9 coding region - by Bioz Stars, 2026-07
    96/100 stars

    Images

    1) Product Images from "Iterative engineering of a compact Cas9 ortholog for in vivo gene editing via single AAV delivery"

    Article Title: Iterative engineering of a compact Cas9 ortholog for in vivo gene editing via single AAV delivery

    Journal: bioRxiv

    doi: 10.64898/2026.01.12.698990

    (A) Table of the Cas9 orthologs utilized in this work, showing their size (amino acid) and PAM sequence (5’). (B) Schematic size comparisons of the panel of Cas9 orthologs tested with SpCas9 and other compact CRISPR systems commonly used for in vivo genome editing. (C) Schematic outlining the experimental steps to evaluate Cas9 ortholog editing efficiency by tdTomato knock-out (KO), quantified by flow cytometry analysis. PiggyBac constructs encode for: ortholog-specific hU6-sgRNA cassettes and puromycin selection gene; doxycycline-inducible Cas9 orthologs and hygromycin selection gene. (D–E) Comparison of SpCas9 cleavage efficiency with Cco, Cme2, Gsp, Msc, Nsp, Sdo and Tmo Cas9 orthologs using three ortholog-specific spacer sequences targeting the tdTomato gene in mESC (D) and HEK293T cells (E). Editing efficiencies at individual target sites are shown in the left panels, while mean editing efficiencies for each Cas9 ortholog across the three sites are presented in the right panels. (F) Heatmaps showing editing efficiencies of Cme2 (left) and Msc (right) using twelve distinct sgRNAs, quantified by flow cytometry. Asterisks indicate P-values from unpaired t -test comparing three independent biological replicates to the respective controls. *P < 0.05. Error bars ± SD.
    Figure Legend Snippet: (A) Table of the Cas9 orthologs utilized in this work, showing their size (amino acid) and PAM sequence (5’). (B) Schematic size comparisons of the panel of Cas9 orthologs tested with SpCas9 and other compact CRISPR systems commonly used for in vivo genome editing. (C) Schematic outlining the experimental steps to evaluate Cas9 ortholog editing efficiency by tdTomato knock-out (KO), quantified by flow cytometry analysis. PiggyBac constructs encode for: ortholog-specific hU6-sgRNA cassettes and puromycin selection gene; doxycycline-inducible Cas9 orthologs and hygromycin selection gene. (D–E) Comparison of SpCas9 cleavage efficiency with Cco, Cme2, Gsp, Msc, Nsp, Sdo and Tmo Cas9 orthologs using three ortholog-specific spacer sequences targeting the tdTomato gene in mESC (D) and HEK293T cells (E). Editing efficiencies at individual target sites are shown in the left panels, while mean editing efficiencies for each Cas9 ortholog across the three sites are presented in the right panels. (F) Heatmaps showing editing efficiencies of Cme2 (left) and Msc (right) using twelve distinct sgRNAs, quantified by flow cytometry. Asterisks indicate P-values from unpaired t -test comparing three independent biological replicates to the respective controls. *P < 0.05. Error bars ± SD.

    Techniques Used: Sequencing, CRISPR, In Vivo, Knock-Out, Flow Cytometry, Construct, Selection, Comparison



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    spcas9 coding region  (Addgene inc)
    96
    Addgene inc spcas9 coding region
    (A) Table of the Cas9 orthologs utilized in this work, showing their size (amino acid) and PAM sequence (5’). (B) Schematic size comparisons of the panel of Cas9 orthologs tested with <t>SpCas9</t> and other compact CRISPR systems commonly used for in vivo genome editing. (C) Schematic outlining the experimental steps to evaluate Cas9 ortholog editing efficiency by tdTomato knock-out (KO), quantified by flow cytometry analysis. PiggyBac constructs encode for: ortholog-specific hU6-sgRNA cassettes and puromycin selection gene; doxycycline-inducible Cas9 orthologs and hygromycin selection gene. (D–E) Comparison of SpCas9 cleavage efficiency with Cco, Cme2, Gsp, Msc, Nsp, Sdo and Tmo Cas9 orthologs using three ortholog-specific spacer sequences targeting the tdTomato gene in mESC (D) and HEK293T cells (E). Editing efficiencies at individual target sites are shown in the left panels, while mean editing efficiencies for each Cas9 ortholog across the three sites are presented in the right panels. (F) Heatmaps showing editing efficiencies of Cme2 (left) and Msc (right) using twelve distinct sgRNAs, quantified by flow cytometry. Asterisks indicate P-values from unpaired t -test comparing three independent biological replicates to the respective controls. *P < 0.05. Error bars ± SD.
    Spcas9 Coding Region, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/spcas9+coding+region/bio_rxiv__64898__2026__01__12__698990-197-0-10?v=Addgene+inc
    Average 96 stars, based on 1 article reviews
    spcas9 coding region - by Bioz Stars, 2026-07
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) Table of the Cas9 orthologs utilized in this work, showing their size (amino acid) and PAM sequence (5’). (B) Schematic size comparisons of the panel of Cas9 orthologs tested with SpCas9 and other compact CRISPR systems commonly used for in vivo genome editing. (C) Schematic outlining the experimental steps to evaluate Cas9 ortholog editing efficiency by tdTomato knock-out (KO), quantified by flow cytometry analysis. PiggyBac constructs encode for: ortholog-specific hU6-sgRNA cassettes and puromycin selection gene; doxycycline-inducible Cas9 orthologs and hygromycin selection gene. (D–E) Comparison of SpCas9 cleavage efficiency with Cco, Cme2, Gsp, Msc, Nsp, Sdo and Tmo Cas9 orthologs using three ortholog-specific spacer sequences targeting the tdTomato gene in mESC (D) and HEK293T cells (E). Editing efficiencies at individual target sites are shown in the left panels, while mean editing efficiencies for each Cas9 ortholog across the three sites are presented in the right panels. (F) Heatmaps showing editing efficiencies of Cme2 (left) and Msc (right) using twelve distinct sgRNAs, quantified by flow cytometry. Asterisks indicate P-values from unpaired t -test comparing three independent biological replicates to the respective controls. *P < 0.05. Error bars ± SD.

    Journal: bioRxiv

    Article Title: Iterative engineering of a compact Cas9 ortholog for in vivo gene editing via single AAV delivery

    doi: 10.64898/2026.01.12.698990

    Figure Lengend Snippet: (A) Table of the Cas9 orthologs utilized in this work, showing their size (amino acid) and PAM sequence (5’). (B) Schematic size comparisons of the panel of Cas9 orthologs tested with SpCas9 and other compact CRISPR systems commonly used for in vivo genome editing. (C) Schematic outlining the experimental steps to evaluate Cas9 ortholog editing efficiency by tdTomato knock-out (KO), quantified by flow cytometry analysis. PiggyBac constructs encode for: ortholog-specific hU6-sgRNA cassettes and puromycin selection gene; doxycycline-inducible Cas9 orthologs and hygromycin selection gene. (D–E) Comparison of SpCas9 cleavage efficiency with Cco, Cme2, Gsp, Msc, Nsp, Sdo and Tmo Cas9 orthologs using three ortholog-specific spacer sequences targeting the tdTomato gene in mESC (D) and HEK293T cells (E). Editing efficiencies at individual target sites are shown in the left panels, while mean editing efficiencies for each Cas9 ortholog across the three sites are presented in the right panels. (F) Heatmaps showing editing efficiencies of Cme2 (left) and Msc (right) using twelve distinct sgRNAs, quantified by flow cytometry. Asterisks indicate P-values from unpaired t -test comparing three independent biological replicates to the respective controls. *P < 0.05. Error bars ± SD.

    Article Snippet: SpCas9 coding region and sgRNA cassette were amplified from pX330-gRNA (Addgene #158973).

    Techniques: Sequencing, CRISPR, In Vivo, Knock-Out, Flow Cytometry, Construct, Selection, Comparison